Stem Cell to Hepatocyte Differentiation Sensor Project

Person

• Ryla Cantorgenali

Purpose

Use Sendai-based microRNA OFF-gate sensors and single-cell sequencing to study differentiation trajectories from stem cells to hepatocytes.

Questions

• Why do some cells get stuck during differentiation?
• When do stem cells acquire canonical interferon responses typical of differentiated cells?

TODO

• Add sensor design, color logic, target miRNAs, differentiation protocol, scRNA-seq plan, and analysis workflow.